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Enhanced stability and activity of an antimicrobial peptide in conjugation with silver nanoparticle

Pal, Indrani and Brahmkhatri, Varsha P and Bera, Swapna and Bhattacharyya, Dipita and Quirishi, Yasrib and Bhunia, Anirban and Atreya, Hanudatta S (2016) Enhanced stability and activity of an antimicrobial peptide in conjugation with silver nanoparticle. In: JOURNAL OF COLLOID AND INTERFACE SCIENCE, 483 . pp. 385-393.

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Official URL: http://dx.doi.org/10.1016/j.jcis.2016.08.043


The conjugation of nanoparticles with antimicrobial peptides (AMP) is emerging as a promising route to achieve superior antimicrobial activity. However, the nature of peptide-nanoparticle interactions in these systems remains unclear. This study describes a system consisting of a cysteine containing antimicrobial peptide conjugated with silver nanoparticles, in which the two components exhibit a dynamic interaction resulting in a significantly enhanced stability and biological activity compared to that of the individual components. This was investigated using NMR spectroscopy in conjunction with other biophysical techniques. Using fluorescence assisted cell sorting and membrane mimics we carried out a quantitative comparison of the activity of the AMP-nanoparticle system and the free peptide. Taken together, the study provides new insights into nanoparticle-AMP interactions at a molecular level and brings out the factors that will be useful for consideration while designing new conjugates with enhanced functionality. (C) 2016 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 03 Dec 2016 04:52
Last Modified: 05 Nov 2018 09:55
URI: http://eprints.iisc.ac.in/id/eprint/55211

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