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Fluorescence Intensity Enhancement Mechanism in Presence of Plasmonic Nanoparticles

Das, Sumana and Javvaji, Brahmanandam and Villa, Krishna Harika and Arikady, Akshata and Hegde, Gopalkrishna M and Mahapatra, Roy D (2016) Fluorescence Intensity Enhancement Mechanism in Presence of Plasmonic Nanoparticles. In: Conference on Plasmonics in Biology and Medicine XIII, FEB 15-16, 2016, San Francisco, CA.

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Official URL: http://dx.doi.org/10.1117/12.2217940


Plasmonic nanoparticles have several applications ranging from catalysis to super-resolution imaging and information storage. Maximum density of optical states is confined on the nanoparticle surface, which are collectively excited by electromagnetic wave and are called surface plasmons. Using nanoparticle based plasmonic interaction with biological cells in an optical fiber integrated microfluidic chip, we show enhancement of fluorescence intensity. Signal from in situ imaging is analyzed with various controls to understand the mechanism. The present study is focused on nanoparticle interaction with cells and on optimization strategies to maximize the fluorescence enhancement at the vicinity of the nanoparticles, for important applications such as fluorescence-based biochip platforms. Result is also correlated ZnO nanoparticle effect on fluorescence enhancement, which has different optoelectronic properties compared to gold nanoparticles. Electromagnetic wave field model is employed to simulate the effect of gold and ZnO nanoparticle on cell with the assumption that the nanoparticles are a collection of discrete dipoles, which are ordered with the fluorescence molecules on cell wall. Simulation model shows enhancement of fluorescence intensity is occurred in presence of gold nanoparticles rather than ZnO nanoparticles, which is confirmed with experimental data.

Item Type: Conference Proceedings
Series.: Proceedings of SPIE
Additional Information: Copy right for this article belongs to the SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Department/Centre: Division of Mechanical Sciences > Aerospace Engineering(Formerly Aeronautical Engineering)
Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited: 28 Oct 2016 07:31
Last Modified: 28 Oct 2016 07:31
URI: http://eprints.iisc.ac.in/id/eprint/55180

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