Surface Engineered Protein Nanoparticles With Hyaluronic Acid Based Multilayers For Targeted Delivery Of Anticancer Agents

Pulakkat, Sreeranjini and Balaji, Sai A and Rangarajan, Annapoorni and Raichur, Ashok M (2016) Surface Engineered Protein Nanoparticles With Hyaluronic Acid Based Multilayers For Targeted Delivery Of Anticancer Agents. In: ACS APPLIED MATERIALS & INTERFACES, 8 (36). pp. 23437-23449.

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Abstract

Layer-by-layer (LbL) technique was employed to modify the surface of doxorubicin (Dox)-loaded bovine serum albumin (BSA) nanoparticles using hyaluronic acid (HA) to enable targeted delivery, to overexpressed CD44, receptors in metastatic breast cancer cells. LbL technique offers a versatile approach to modify the surface of colloidal nanoparticles without any covalent modification. Dox-loaded BSA (Dox Ab) nanoparticles optimized for their size, zeta potential, and drug encapsulation efficiency were prepared by modified desolvation technique. The cellular uptake and cytotoxicity of the coated Dox Ab nanoparticles were analyzed in CD44 overexpressing breast cancer cell line MDA-MB-231. Nanoparticles with HA as the, final layer (Dox Ab HA) showed maximum cellular uptake in MDA-MB-231 owing to the CD44 receptor-mediated endocytosis and hence, exhibited more cytotoxicity as compared to free Dox. Further, luciferase-transfected MDA-MB-231 cells were used to induce tumor in BALB/c female nude mice to enable whole body tumor imaging. The mice were imaged before and after Dox treatment to visualize the tumor growth. The in vivo biodistribution of Dox Ab HA nanoparticles in nude mice showed maximum accumulation in tumor, and importantly, better tumor reduction in. comparison with free Dox, thus paving the way for improved drug delivery into tumors.

Item Type:	Journal Article
Publication:	ACS APPLIED MATERIALS & INTERFACES
Additional Information:	Copy right for this article belongs to the AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	28 Oct 2016 07:15
Last Modified:	28 Oct 2016 07:15
URI:	http://eprints.iisc.ac.in/id/eprint/55146

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