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Network analysis reveals common host protein/s modulating pathogenesis of neurotropic viruses

Ghosh, Sourish and Mukherjee, Sriparna and Sengupta, Nabonita and Roy, Arunava and Dey, Dhritiman and Chakraborty, Surajit and Chattopadhyay, Dhrubajyoti and Banerjee, Arpan and Basu, Anirban (2016) Network analysis reveals common host protein/s modulating pathogenesis of neurotropic viruses. In: SCIENTIFIC REPORTS, 6 .

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Official URL: http://dx.doi.org/10.1038/srep32593


Network analysis through graph theory provides a quantitative approach to characterize specific proteins and their constituent assemblies that underlie host-pathogen interactions. In the present study, graph theory was used to analyze the interactome designed out of 50 differentially expressing proteins from proteomic analysis of Chandipura Virus (CHPV, Family: Rhabdoviridae) infected mouse brain tissue to identify the primary candidates for intervention. Using the measure of degree centrality, that quantifies the connectedness of a single protein within a milieu of several other interacting proteins, DJ-1 was selected for further molecular validation. To elucidate the generality of DJ-1's role in propagating infection its role was also monitored in another RNA virus, Japanese Encephalitis Virus (JEV, Family: Flaviviridae) infection. Concurrently, DJ-1 got over-expressed in response to reactive oxygen species (ROS) generation following viral infection which in the early phase of infection migrated to mitochondria to remove dysfunctional mitochondria through the process of mitophagy. DJ-1 was also observed to modulate the viral replication and interferon responses along with low-density lipoprotein (LDL) receptor expression in neurons. Collectively these evidences reveal a comprehensive role for DJ-1 in neurotropic virus infection in the brain.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 22 Oct 2016 09:20
Last Modified: 22 Oct 2016 09:20
URI: http://eprints.iisc.ac.in/id/eprint/55013

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