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Transcriptional regulation of topology modulators and transcription regulators of Mycobacterium tuberculosis

Ghosh, Soumitra and Padmanabhan, Bhavna and Godbole, Adwait Anand and Tare, Priyanka and Ahmed, Wareed and Vasu, Kommireddy and China, Arnab and Kumar, Rupesh and Mitra, Anirban and Nagaraja, Valakunja (2016) Transcriptional regulation of topology modulators and transcription regulators of Mycobacterium tuberculosis. In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 475 (3). pp. 257-263.

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2016.05.078


Mycobacterium tuberculosis (Mtb) is a formidable pathogen which has the ability to survive the hostile environment of the host by evading the host defense system. The re-configuration of its transcriptional and metabolic process allows the pathogen to confront the adverse environment within the host macrophages. The factors that assist the transcription and modulate the DNA topology would have to play a key role in the regulation of global gene expression of the organism. How transcription of these essential housekeeping genes alters in response to growth conditions and environmental stress has not been addressed together in a set of experimental conditions in Mtb. Now, we have mapped the transcription start sites (TSS) and promoters of several genes that play a central role in the regulation of DNA topology and transcription in Mtb. Using in vivo reporter assays, we validated the activity of the identified promoter elements in different growth conditions. The variation in transcript abundance of these essential genes was also analyzed in growth phase-dependent manner. These data provide the first glimpse into the specific adaptive changes in the expression of genes involved in transcription and DNA topology modulation in Mtb. (C) 2016 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
Keywords: Mycobacterium; Promoter; Transcription; Topology
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 19 Jul 2016 09:08
Last Modified: 19 Jul 2016 09:08
URI: http://eprints.iisc.ac.in/id/eprint/54180

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