Manchineella, Shivaprasad and Thrivikraman, Greeshma and Khanum, Khadija K and Ramamurthy, Praveen C and Basu, Bikramjit and Govindaraju, T (2016) Pigmented Silk Nanofibrous Composite for Skeletal Muscle Tissue Engineering. In: ADVANCED HEALTHCARE MATERIALS, 5 (10). pp. 1222-1232.
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Skeletal muscle tissue engineering (SMTE) employs designed biomaterial scaffolds for promoting myogenic differentiation of myoblasts to functional myotubes. Oxidative stress plays a significant role in the biocompatibility of biomaterials as well as in the fate of myoblasts during myogenesis and is also associated with pathological conditions such as myotonic dystrophy. The inherent electrical excitability of muscle cells inspired the use of electroactive scaffolds for SMTE. Conducting polymers attracted the attention of researchers for their use in muscle tissue engineering. However, poor biocompatibility, biodegradability and development of oxidative stress associated immunogenic response limits the extensive use of synthetic conducting polymers for SMTE. In order to address the limitations of synthetic polymers, intrinsically electroactive and antioxidant silk fibroin/melanin composite films and electrospun fiber mats were fabricated and evaluated as scaffolds for promoting myogenesis in vitro. Melanin incorporation modulated the thermal stability, electrical conductivity of scaffolds, fiber alignment in electrospun mats and imparted good antioxidant properties to the scaffolds. The composite electrospun scaffolds promoted myoblast assembly and differentiation into uniformly aligned high aspect ratio myotubes. The results highlight the significance of scaffold topography along with conductivity in promoting myogenesis and the potential application of silk nanofibrous composite as electoractive platform for SMTE.
| Item Type: | Journal Article |
|---|---|
| Publication: | ADVANCED HEALTHCARE MATERIALS |
| Publisher: | WILEY-BLACKWELL |
| Additional Information: | Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA |
| Keywords: | biomaterials; electroactive and antioxidant scaffolds; electrospinning; myogenesis; silk; melanin composite |
| Department/Centre: | Division of Chemical Sciences > Materials Research Centre Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy) |
| Date Deposited: | 08 Jul 2016 06:42 |
| Last Modified: | 08 Jul 2016 06:42 |
| URI: | http://eprints.iisc.ac.in/id/eprint/54152 |
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