Generation of Catalytic Antibodies Is an Intrinsic Property of an Individual's Immune System: A Study on a Large Cohort of Renal Transplant Patients

Mahendra, Ankit and Peyron, Ivan and Thaunat, Olivier and Dollinger, Cecile and Gilardin, Laurent and Sharma, Meenu and Wootla, Bharath and Rao, Desirazu N and Padiolleau-Lefevre, Severine and Boquet, Didier and More, Abhijit and Varadarajan, Navin and Kaveri, Srini V and Legendre, Christophe and Lacroix-Desmazes, Sebastien (2016) Generation of Catalytic Antibodies Is an Intrinsic Property of an Individual's Immune System: A Study on a Large Cohort of Renal Transplant Patients. In: JOURNAL OF IMMUNOLOGY, 196 (10). pp. 4075-4081.

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Abstract

Renal transplant is the treatment of choice for patients with terminal end-stage renal disease. We have previously identified low levels of catalytic IgG as a potential prognosis marker for chronic allograft rejection. The origin and physiopathological relevance of catalytic Abs is not well understood, owing to the fact that catalytic Abs have been studied in relatively small cohorts of patients with rare diseases and/or without systematic follow-up. In the current study, we have followed the evolution of the levels of catalytic IgG in a large cohort of renal transplant patients over a 2-y period. Our results demonstrate that, prior to transplant, patients with renal failure present with heterogeneous levels of IgG hydrolyzing the generic proline-phenylalanine-arginine-methylcoumarinamide (PFR-MCA) substrate. PFR-MCA hydrolysis was greater for patients' IgG than for a therapeutic preparation of pooled IgG from healthy donors. Renal transplant was marked by a drastic decrease in levels of catalytic IgG over 3 mo followed by a steady increase during the next 21 mo. Patients who displayed high levels of catalytic IgG pretransplant recovered high levels of catalytic Abs 2 y posttransplant. Interestingly, IgG-mediated hydrolysis of a model protein substrate, procoagulant factor VIII, did not correlate with that of PFR-MCA prior transplantation, whereas it did 12 mo posttransplant. Taken together, our results suggest that the level of circulating catalytic IgG under pathological conditions is an intrinsic property of each individual's immune system and that recovery of pretransplant levels of catalytic IgG is accompanied by changes in the repertoire of target Ags.

Item Type:	Journal Article
Publication:	JOURNAL OF IMMUNOLOGY
Publisher:	AMER ASSOC IMMUNOLOGISTS
Additional Information:	Copy right for this article belongs to the AMER ASSOC IMMUNOLOGISTS, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	15 Jun 2016 06:20
Last Modified:	15 Jun 2016 06:20
URI:	http://eprints.iisc.ac.in/id/eprint/53953

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