A novel viral RNA helicase with an independent translation enhancement activity

Abraham, Ambily and Savithri, Handanahal S (2016) A novel viral RNA helicase with an independent translation enhancement activity. In: FEBS LETTERS, 590 (8). pp. 1187-1199.

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Abstract

RNA helicases have not been identified among negative sense RNA viruses. In this study, it is shown that Nonstructural protein (NSs) of Groundnut bud necrosis virus (GBNV) acts as a Mg2+- and ATP-dependent bipolar RNA helicase. Biophysical and biochemical analysis of the deletion mutants (N Delta 124 NSs, C Delta 80 NSs) revealed that both the N- and C-terminal residues are required for substrate binding, oligomerization and helicase activity, but are dispensable for ATPase activity. Interestingly, NSs could enhance the translation of RNA (similar to 10-fold) independent of its helicase activity. This is the first report of a RNA helicase from negative strand RNA viruses.

Item Type:	Journal Article
Publication:	FEBS LETTERS
Publisher:	WILEY-BLACKWELL
Additional Information:	Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Keywords:	bipolar RNA helicase; Groundnut bud necrosis virus; nonstructural protein; translation enhancer
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	11 Jun 2016 07:52
Last Modified:	11 Jun 2016 07:52
URI:	http://eprints.iisc.ac.in/id/eprint/53906

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