Raman and infra-red microspectroscopy: towards quantitative evaluation for clinical research by ratiometric analysis

Kumar, Srividya and Verma, Taru and Mukherjee, Ria and Ariese, Freek and Somasundaram, Kumaravel and Umapathy, Siva (2016) Raman and infra-red microspectroscopy: towards quantitative evaluation for clinical research by ratiometric analysis. In: CHEMICAL SOCIETY REVIEWS, 45 (7). pp. 1879-1900.

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Abstract

Biomolecular structure elucidation is one of the major techniques for studying the basic processes of life. These processes get modulated, hindered or altered due to various causes like diseases, which is why biomolecular analysis and imaging play an important role in diagnosis, treatment prognosis and monitoring. Vibrational spectroscopy (IR and Raman), which is a molecular bond specific technique, can assist the researcher in chemical structure interpretation. Based on the combination with microscopy, vibrational microspectroscopy is currently emerging as an important tool for biomedical research, with a spatial resolution at the cellular and sub-cellular level. These techniques offer various advantages, enabling label-free, biomolecular fingerprinting in the native state. However, the complexity involved in deciphering the required information from a spectrum hampered their entry into the clinic. Today with the advent of automated algorithms, vibrational microspectroscopy excels in the field of spectropathology. However, researchers should be aware of how quantification based on absolute band intensities may be affected by instrumental parameters, sample thickness, water content, substrate backgrounds and other possible artefacts. In this review these practical issues and their effects on the quantification of biomolecules will be discussed in detail. In many cases ratiometric analysis can help to circumvent these problems and enable the quantitative study of biological samples, including ratiometric imaging in 1D, 2D and 3D. We provide an extensive overview from the recent scientific literature on IR and Raman band ratios used for studying biological systems and for disease diagnosis and treatment prognosis.

Item Type:	Journal Article
Publication:	CHEMICAL SOCIETY REVIEWS
Publisher:	ROYAL SOC CHEMISTRY
Additional Information:	Copy right for this article belongs to the ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Chemical Sciences > Inorganic & Physical Chemistry Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	28 Apr 2016 05:02
Last Modified:	28 Apr 2016 05:02
URI:	http://eprints.iisc.ac.in/id/eprint/53747

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