Enzymatic degradation of polycaprolactone-gelatin blend

Banerjee, Aditi and Chatterjee, Kaushik and Madras, Giridhar (2016) Enzymatic degradation of polycaprolactone-gelatin blend. In: MATERIALS RESEARCH EXPRESS, 2 (4).

PDF Mat_Res_Exp_2-4_045303_2016.pdf - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

Blends of polycaprolactone (PCL), a synthetic polymer and gelatin, natural polymer offer a optimal combination of strength, water wettability and cytocompatibility for use as a resorbable biomaterial. The enzymatic degradation of PCL, gelatin and PCL-gelatin blended films was studied in the presence of lipase (Novozym 435, immobilized) and lysozyme. Novozym 435 degraded the PCL films whereas lysozyme degraded the gelatin. Though Novozym 435 and lysozyme individually could degrade PCL-gelatin blended films, the combination of these enzymes showed the highest degradation of these blended films. Moreover, the enzymatic degradation was much faster when fresh enzymes were added at regular intervals. The changes in physico-chemical properties of polymer films due to degradation were studied by scanning electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. These results have important implications for designing resorbable biomedical implants.

Item Type:	Journal Article
Publication:	MATERIALS RESEARCH EXPRESS
Publisher:	IOP PUBLISHING LTD
Additional Information:	Copy right for this article belongs to the IOP PUBLISHING LTD, TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND
Keywords:	polymer blend; degradation; enzyme kinetics
Department/Centre:	Division of Mechanical Sciences > Chemical Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	26 Mar 2016 05:52
Last Modified:	26 Mar 2016 05:52
URI:	http://eprints.iisc.ac.in/id/eprint/53431

Actions (login required)

View Item