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Enhancement Mechanism of Fluorescence Intensity in Presence of Plasmonic Nanoparticles

Das, Sumana and Vasireddi, Ramakrishna and Villa, Krishna Harika and Javvaji, Brahmanandam and Hedge, Gopalkrishna M and Mahapatra, Roy D (2015) Enhancement Mechanism of Fluorescence Intensity in Presence of Plasmonic Nanoparticles. In: Conference on Biophotonics Japan, OCT 27-28, 2015, Tokyo, JAPAN .

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The emission intensity of fluorophore molecule may change in presence of strong plasmon field induced by nanoparticles. The enhancement intensity is optimized through selective clustering or functionalization of nanoparticles in closed vicinity of fluorophore. Our study is aimed at understanding the enhancement mechanism of fluorescence intensity in presence of gold nanoparticles to utilize it in molecular sensing and in situ imaging in the microfluidic lab-on-chip device. Related phenomena are studied in situ in a microfluidic channel via fluorescence imaging. Detailed analysis is carried out to understand the possible mechanism of enhancement of fluorescence due to nanoparticles. In the present experimental study we show that SYTO9 fluorescence intensity increased in presence of Au nanoparticles of similar to 20 nm diameter. The fluorescence intensity is 20 time more compared to that in absence of Au nanoparticles. The enhancement of fluorescence intensity is attributed to the plasmonic resonance of Au nanoparticle at around the fluorescence emission wavelength. Underlying fundamental mechanism via dipole interaction model is explored for quantitative correlation of plasmonic enhancement properties.

Item Type: Conference Proceedings
Series.: Proceedings of SPIE
Additional Information: Copy right for this article belongs to the SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Keywords: nanoparticle; plasmon; resonance; fluorescence; optical transmission; dipole
Department/Centre: Division of Mechanical Sciences > Aerospace Engineering(Formerly Aeronautical Engineering)
Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited: 29 Feb 2016 06:49
Last Modified: 29 Feb 2016 06:49
URI: http://eprints.iisc.ac.in/id/eprint/53340

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