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Limited-view light sheet fluorescence microscopy for three dimensional volume imaging

Rasmi, CK and Mohan, Kavya and Madhangi, M and Rajan, K and Nongthomba, U and Mondal, Partha P (2016) Limited-view light sheet fluorescence microscopy for three dimensional volume imaging. In: APPLIED PHYSICS LETTERS, 107 (26).

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Official URL: http://dx.doi.org/10.1063/1.4938536


We propose and demonstrate a limited-view light sheet microscopy (LV-LSM) for three dimensional (3D) volume imaging. Realizing that longer and frequent image acquisition results in significant photo-bleaching, we have taken limited angular views (18 views) of the macroscopic specimen and integrated with maximum likelihood (ML) technique for reconstructing high quality 3D volume images. Existing variants of light-sheet microscopy require both rotation and translation with a total of approximately 10-fold more views to render a 3D volume image. Comparatively, LV-LSM technique reduces data acquisition time and consequently minimizes light-exposure by many-folds. Since ML is a post-processing technique and highly parallelizable, this does not cost precious imaging time. Results show noise-free and high contrast volume images when compared to the state-of-the-art selective plane illumination microscopy. (C) 2015 AIP Publishing LLC.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the AMER INST PHYSICS, 1305 WALT WHITMAN RD, STE 300, MELVILLE, NY 11747-4501 USA
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Division of Physical & Mathematical Sciences > Physics
Date Deposited: 18 Feb 2016 05:24
Last Modified: 18 Feb 2016 05:24
URI: http://eprints.iisc.ac.in/id/eprint/53285

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