Molecular profiling of sepsis in mice using Fourier Transform Infrared Microspectroscopy

Gautam, Rekha and Deobagkar-Lele, Mukta and Majumdar, Shamik and Chandrasekar, Bhagawat and Victor, Emmanuel and Ahmed, Syed Moiz and Wadhwa, Nitin and Verma, Taru and Kumar, Srividya and Sundaresan, Nagalingam Ravi and Umapathy, Siva and Nandi, Dipankar (2016) Molecular profiling of sepsis in mice using Fourier Transform Infrared Microspectroscopy. In: JOURNAL OF BIOPHOTONICS, 9 (1-2). pp. 67-82.

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Abstract

Sepsis is a life threatening condition resulting from a high burden of infection. It is a major health care problem and associated with inflammation, organ dysfunction and significant mortality. However, proper understanding and delineating the changes that occur during this complex condition remains a challenge. A comparative study involving intra-peritoneal injection of BALB/c mice with Salmonella Typhimurium (infection), lipopolysaccharide (endotoxic shock) or thioglycollate (sterile peritonitis) was performed. The changes in organs and sera were profiled using immunological assays and Fourier Transform Infrared (FTIR) micro-spectroscopy. There is a rapid rise in inflammatory cytokines accompanied with lowering of temperature, respiratory rate and glucose amounts in mice injected with S. Typhimurium or lipopolysaccharide. FTIR identifies distinct changes in liver and sera: decrease in glycogen and protein/lipid ratio and increase in DNA and cholesteryl esters. These changes were distinct from the pattern observed in mice treated with thioglycollate and the differences in the data obtained between the three models are discussed. The combination of FTIR spectroscopy and other biomarkers will be valuable in monitoring molecular changes during sepsis. GRAPHICS] Intra-peritoneal infection with high dose of Salmonella Typhimurium leads to rapid increase in inflammatory cytokines, e.g. Tnf alpha (A). FTIR analysis of liver (B) and sera (C) identifies several metabolic changes: glycogen, protein/lipid, cholesteryl esters and DNA.

Item Type:	Journal Article
Publication:	JOURNAL OF BIOPHOTONICS
Publisher:	WILEY-V C H VERLAG GMBH
Additional Information:	Copy right for this article belongs to the WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY
Keywords:	Fourier Transform Infrared Spectroscopy; imaging; infection; inflammation; lipopolysaccharide; sepsis
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Biological Sciences > Microbiology & Cell Biology Division of Chemical Sciences > Inorganic & Physical Chemistry Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	10 Feb 2016 05:45
Last Modified:	04 Feb 2019 10:10
URI:	http://eprints.iisc.ac.in/id/eprint/53202

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