Role of spacer length in interaction between novel gemini imidazolium surfactants and Rhizopus oryzae lipase

Adak, Sunita and Datta, Sougata and Bhattacharya, Santanu and Banerjee, Rintu (2015) Role of spacer length in interaction between novel gemini imidazolium surfactants and Rhizopus oryzae lipase. In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 81 . pp. 560-567.

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Abstract

An insight into the effects of new ionic liquid-type gemini imidazolium cationic surfactants on the structure and function of the lipases is of prime importance for their potential application. Changes in the activity, stability and structure of Rhizopus ouzae lipase in the presence of novel gemini surfactants, C-16-3-C(16)im]Br-2 and C-16-12-C(16)im]Br-2 were probed in the present study. Surfactant with shorter spacer length, C-16-3-C(i6)im]Br-2 was found to be better in improving the hydrolytic activity and thermal stability of the lipase. For both the surfactants, activation was concentration dependent. CD spectroscopy results showed a decrease in a-helix and an increase in beta-sheet content in the presence of these surfactants. A higher structural change observed in presence of C-16-12-C(16)im]Br-2 correlated with lower enzyme activity. Isothermal titration calorimetric studies showed the binding to be spontaneous in nature based on sequential two site binding model. The forces involved in binding were found to differ for the two surfactants proving that the spacer length is an important factor which governs the interaction. These surfactants could be used as promising components both in enzyme modification and media engineering for attaining the desired goals in biocatalytic reactions. (C) 2015 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	Copy right for this article belongs to the ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Keywords:	Lipase; Gemini imidazolium surfactant; Activity; Stability; Structural study; Interactions
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	27 Jan 2016 06:12
Last Modified:	27 Jan 2016 06:12
URI:	http://eprints.iisc.ac.in/id/eprint/53184

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