Govindaraj, Vijayakumar and Rao, Jagannadha A (2015) Comparative proteomic analysis of primordial follicles from ovaries of immature and aged rats. In: SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE, 61 (6). pp. 367-375.
Full text not available from this repository. (Request a copy)Abstract
Age related decline in reproductive performance in women is well documented and apoptosis has been considered as one of the reasons for the decline of primordial follicle reserve. Recently we observed a decline in the efficiency of DNA repair ability in aged rat primordial follicles as demonstrated by decreased mRNA levels of DNA repair genes BRCA1 and H2AX. In the present study, a two-dimensional electrophoresis (2DE) proteomic approach was employed to identify differentially expressed proteins in primordial follicles isolated from ovaries of immature (approximate to 20 days) and aged (approximate to 400-450 days) rats. Using MALDI-TOF/TOF MS, we identified 13 differentially expressed proteins (p<0.05) which included seven up-regulated and six down-regulated proteins in aged primordial follicles. These proteins are involved in a wide range of biological functions including apoptosis, DNA repair, and the immune system. Interestingly, the differentially expressed proteins such as FIGNL1 (DNA repair) and BOK (apoptotic protein) have not been previously reported in the rat primordial follicles and these proteins can be related to some common features of ovarian aging such as loss of follicle reserve and genome integrity. The quantitative differences of two important proteins BOK and FIGNL1 observed by the proteomic analysis were correlated with the transcript levels, as determined by semi-quantitative RT-PCR. Our results improve the current knowledge about protein factors associated with molecular changes in rat primordial follicles as a function of aging and our understanding of the proteomic processes involved in degenerative changes observed in aging primordial follicles.
| Item Type: | Journal Article |
|---|---|
| Publication: | SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE |
| Publisher: | TAYLOR & FRANCIS INC |
| Additional Information: | Copy right for this article belongs to the TAYLOR & FRANCIS INC, 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA |
| Keywords: | 2DE; aging; BOK; Fignl1; oocytes; primordial follicles |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 10 Dec 2015 06:01 |
| Last Modified: | 10 Dec 2015 06:01 |
| URI: | http://eprints.iisc.ac.in/id/eprint/52875 |
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