A comprehensive analysis of the chorion locus in silkmoth

Chen, Zhiwei and Nohata, Junko and Guo, Huizhen and Li, Shenglong and Liu, Jianqiu and Guo, Youbing and Yamamoto, Kimiko and Kadono-Okuda, Keiko and Liu, Chun and Arunkumar, Kallare P and Nagaraju, Javaregowda and Zhang, Yan and Liu, Shiping and Labropoulou, Vassiliki and Swevers, Luc and Tsitoura, Panagiota and Iatrou, Kostas and Gopinathan, Karumathil P and Goldsmith, Marian R and Xia, Qingyou and Mita, Kazuei (2015) A comprehensive analysis of the chorion locus in silkmoth. In: Scientific Reports, 5 .

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Abstract

Despite more than 40 years of intense study, essential features of the silkmoth chorion (eggshell) are still not fully understood. To determine the precise structure of the chorion locus, we performed extensive EST analysis, constructed a bacterial artificial chromosome (BAC) contig, and obtained a continuous genomic sequence of 871,711 base pairs. We annotated 127 chorion genes in two segments interrupted by a 164 kb region with 5 non-chorion genes, orthologs of which were on chorion bearing scaffolds in 4 ditrysian families. Detailed transcriptome analysis revealed expression throughout choriogenesis of most chorion genes originally categorized as ``middle'', and evidence for diverse regulatory mechanisms including cis-elements, alternative splicing and promoter utilization, and antisense RNA. Phylogenetic analysis revealed multigene family associations and faster evolution of early chorion genes and transcriptionally active pseudogenes. Proteomics analysis identified 99 chorion proteins in the eggshell and micropyle localization of 1 early and 6 Hc chorion proteins.

Item Type:	Journal Article
Publication:	Scientific Reports
Publisher:	NATURE PUBLISHING GROUP
Additional Information:	Copy right for this article belongs to the NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	10 Dec 2015 05:59
Last Modified:	10 Dec 2015 05:59
URI:	http://eprints.iisc.ac.in/id/eprint/52872

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