A rapid method to assess reactive oxygen species in yeast using H2DCF-DA

James, Joel and Fiji, Nikita and Roy, Debasish and Andrew, Daniel MG and Shihabudeen, Mohamed Sham and Chattopadhyay, Debarati and Thirumurugan, Kavitha (2015) A rapid method to assess reactive oxygen species in yeast using H2DCF-DA. In: ANALYTICAL METHODS, 7 (20). pp. 8572-8575.

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Abstract

A protocol to efficiently assess Reactive Oxygen Species (ROS) levels in yeast cells using H2DCF-DA is described here. This method employs lithium acetate to permeate the cell wall, and thus, augments the release of the fluorescent product, dichlorofluorescein from the cells. This protocol obviates the need for both physical and enzymatic lysis methods that are arduous and time consuming. This method is simple, less time consuming and reproducible, especially while dealing with a large sample size. The lithium acetate method gave significantly reproducible and linear results (P < 0.0001), as compared with direct measurement (P = 0.0005), sonication (P = 0.1466) and bead beating (P = 0.0028).

Item Type:	Journal Article
Publication:	ANALYTICAL METHODS
Publisher:	ROYAL SOC CHEMISTRY
Additional Information:	Copy right for this article belongs to the ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	05 Nov 2015 08:55
Last Modified:	05 Nov 2015 08:55
URI:	http://eprints.iisc.ac.in/id/eprint/52697

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