NANOLOCALIZED SINGLE CELL MEMBRANE NANOELECTROPORATION

Santra, TS and Lee, CW and Kar, S and Borana, J and Wang, PC and Tseng, FG (2014) NANOLOCALIZED SINGLE CELL MEMBRANE NANOELECTROPORATION. In: 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS), APR 13-16, 2014, Honolulu, HI, pp. 285-288.

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Abstract

Today single cell research is a great interest to analyze cell to cell or cell to environment behavior with their intracellular compounds, where bulk measurement can provide average value. To deliver biomolecules precise and localized way into single living cell with high transfection rate and high cell viability is a challenging and promisible task for biological and therapeutic research. In this report, we present a nano-localized single cell nano-electroporation technique, where electroporation take place in a very precise and localized area on a single cell membrane to achieve high efficient delivery with high cell viability. We fabricated 60nm gap with 40 nm triangular Indium Tin Oxide (ITO) based nano-eletcrode tip, which can intense electric field in a nano-localized area of a single cell to permeabilize cell membrane and deliver exogenous biomolecules from outside to inside of the cell. This device successfully deliver dyes, proteins into single cell with high cell viability (98%). The process not only control precise delivery mechanism into single cell with membrane reversibility, but also it can provide special, temporal and qualitative dosage control, which might be beneficial for therapeutic and biological cell studies.

Item Type:	Conference Proceedings
Publisher:	IEEE
Additional Information:	Copy right for this article belongs to the IEEE, 345 E 47TH ST, NEW YORK, NY 10017 USA
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	09 Oct 2015 05:56
Last Modified:	09 Oct 2015 05:56
URI:	http://eprints.iisc.ac.in/id/eprint/52530

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