Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates

Law-Hine, Didier and Sahoo, Anil K and Bailleux, Virginie and Zeghal, Mehdi and Prevost, Sylvain and Maiti, Prabal K and Bressanelli, Stephane and Constantin, Doru and Tresset, Guillaume (2015) Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates. In: JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 6 (17). pp. 3471-3476.

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Abstract

Viral capsids derived from an icosahedral plant virus widely used in physical and nanotechnological investigations were fully dissociated into dimers by a rapid change of pH. The process was probed in vitro at high spatiotemporal resolution by time-resolved small-angle X-ray scattering using a high brilliance synchrotron source. A powerful custom-made global fitting algorithm allowed us to reconstruct the most likely pathway parametrized by a set of stoichiometric coefficients and to determine the shape of two successive intermediates by ab initio calculations. None of these two unexpected intermediates was previously identified in self-assembly experiments, which suggests that the disassembly pathway is not a mirror image of the assembly pathway. These findings shed new light on the mechanisms and the reversibility of the assembly/disassembly of natural and synthetic virus-based systems. They also demonstrate that both the structure and dynamics of an increasing number of intermediate species become accessible to experiments.

Item Type:	Journal Article
Publication:	JOURNAL OF PHYSICAL CHEMISTRY LETTERS
Publisher:	AMER CHEMICAL SOC
Additional Information:	Copy right for this article belongs to the AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	09 Oct 2015 04:28
Last Modified:	09 Oct 2015 04:28
URI:	http://eprints.iisc.ac.in/id/eprint/52504

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