Single-Cell Optical Absorbance Characterization With High-Throughput Microfluidic Microscopy

Banoth, Earu and Jagannadh, Veerendra Kalyan and Gorthi, Sai Siva (2015) Single-Cell Optical Absorbance Characterization With High-Throughput Microfluidic Microscopy. In: IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 22 (3).

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Abstract

Morphological changes in cells associated with disease states are often assessed using clinical microscopy. However, the changes in chemical composition of cells can also be used to detect disease conditions. Optical absorption measurements carried out on single cells using inexpensive sources, detectors can help assess the chemical composition of cells; thereby enable detection of diseases. In this article, we present a novel technique capable of simultaneously detecting changes in morphology and chemical composition of cells. The presented technique enables characterization of optical absorbance-based methods against microscopy for detection of disease states. Using the technique, we have been able to achieve a throughput of about 1000 cells per second. We demonstrate the proof-of-principle by detecting malaria in a given blood sample. The presented technique is capable of detecting very lower levels of parasitemia within time scales comparable to antigen-based rapid diagnostic tests.

Item Type:	Journal Article
Publication:	IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
Publisher:	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Additional Information:	Copy right for this article belongs to the IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC, 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA
Keywords:	Optical absorbance; single-cell; optofluidics; high-throughput measurement; microfluidic microscopy; imaging flow cytometry
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	24 Sep 2015 06:29
Last Modified:	24 Sep 2015 06:29
URI:	http://eprints.iisc.ac.in/id/eprint/52412

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