Reversible Aptamer-Au Plasmon Rulers for Secreted Single Molecules

Lee, Somin Eunice and Chen, Qian and Bhat, Ramray and Petkiewicz, Shayne and Smith, Jessica M and Ferry, Vivian E and Correia, Ana Luisa and Alivisatos, Paul A and Bissell, Mina J (2015) Reversible Aptamer-Au Plasmon Rulers for Secreted Single Molecules. In: NANO LETTERS, 15 (7). pp. 4564-4570.

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Abstract

Plasmon rulers, consisting of pairs of gold nanoparticles, allow single-molecule analysis without photobleaching or blinking; however, current plasmon rulers are irreversible, restricting detection to only single events. Here, we present a reversible plasmon ruler, comprised of coupled gold nanoparticles linked by a single aptamer, capable of binding individual secreted molecules with high specificity. We show that the binding of target secreted molecules to the reversible plasmon ruler is characterized by single-molecule sensitivity, high specificity, and reversibility. Such reversible plasmon rulers should enable dynamic and adaptive live-cell measurement of secreted single molecules in their local microenvironment.

Item Type:	Journal Article
Publication:	NANO LETTERS
Publisher:	AMER CHEMICAL SOC
Additional Information:	Copy right for this article belongs to the AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Keywords:	plasmon; single-molecule; secreted molecule; imaging; gold nanoparticle
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	11 Aug 2015 07:37
Last Modified:	11 Aug 2015 07:37
URI:	http://eprints.iisc.ac.in/id/eprint/52076

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