Imaging Flow Cytometry With Femtosecond Laser-Micromachined Glass Microfluidic Channels

Jagannadh, Veerendra Kalyan and Mackenzie, Mark D and Pal, Parama and Kar, Ajoy K and Gorthi, Sai Siva (2015) Imaging Flow Cytometry With Femtosecond Laser-Micromachined Glass Microfluidic Channels. In: IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 21 (4).

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Abstract

Microfluidic/optofluidic microscopy is a versatile modality for imaging and analyzing properties of cells/particles while they are in flow. In this paper, we demonstrate the integration of fused silica microfluidics fabricated using femtosecond laser machining into optofluidic imaging systems. By using glass for the sample stage of our microscope, we have exploited its superior optical quality for imaging and bio-compatibility. By integrating these glass microfluidic devices into a custom-built bright field microscope, we have been able to image red blood cells in flow with high-throughputs and good fidelity. In addition, we also demonstrate imaging as well as detection of fluorescent beads with these microfluidic devices.

Item Type:	Journal Article
Publication:	IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
Publisher:	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Additional Information:	Copy right for this article belongs to the IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC, 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA
Keywords:	Ultra-fast laser inscription; microfluidic/optofluidic imaging; high-throughput imaging; glass microfluidics
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	31 Jul 2015 14:43
Last Modified:	23 Oct 2018 14:50
URI:	http://eprints.iisc.ac.in/id/eprint/51988

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