ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis

Jose, Mini and Tollis, Sylvain and Nair, Deepak and Mitteau, Romain and Velours, Christophe and Massoni-Laporte, Aurelie and Royou, Anne and Sibarita, Jean-Baptiste and McCusker, Derek (2015) A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis. In: MOLECULAR BIOLOGY OF THE CELL, 26 (13). pp. 2519-2534.

Full text not available from this repository. (Request a copy)
Official URL: http://dx.doi.org/10.1091/mbc.E14-11-1527


The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-based screen in budding yeast, we identified 89 mutants displaying defects in the localization of either one or both pathways. High-resolution single-vesicle tracking revealed that the endocytic and exocytic mutants she4 Delta and bud6 Delta alter post-Golgi vesicle dynamics in opposite ways. The endocytic and exocytic pathways display strong interdependence during polarity establishment while being more independent during polarity maintenance. Systems analysis identified the exocyst complex as a key network hub, rich in genetic interactions with endocytic and exocytic components. Exocyst mutants displayed altered endocytic and post-Golgi vesicle dynamics and interspersed endocytic and exocytic domains compared with control cells. These data are consistent with an important role for the exocyst in coordinating endocytosis and exocytosis.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the AMER SOC CELL BIOLOGY, 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA
Department/Centre: Division of Biological Sciences > Centre for Neuroscience
Date Deposited: 31 Jul 2015 10:55
Last Modified: 31 Jul 2015 10:55
URI: http://eprints.iisc.ac.in/id/eprint/51947

Actions (login required)

View Item View Item