The crystal structure of a lectin from Butea monosperma: Insight into its glycosylation and binding of ligands

Abhilash, Joseph and Geethanandan, K and Bharath, SR and Sabu, A and Sadasivan, C and Haridas, M (2015) The crystal structure of a lectin from Butea monosperma: Insight into its glycosylation and binding of ligands. In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 72 . pp. 1376-1383.

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Abstract

Crystal structure of a lectin purified from Butea monosperma seeds was determined by Molecular Replacement method. Its primary structure was determined by Tandem Mass Spectroscopy and electron density maps from X-ray diffraction data. Its quaternary structure was tetrameric, formed of two monomers, alpha and beta, beta appearing as truncated alpha. The occurrence of two tetramers in the asymmetric unit of the crystal might be a consequence of asymmetric contacts due to difference in glycosylation and variable loops structures, to form an `octamer-structure'. The crystal structure showed binding pockets for gamma Abu, having a proposed role in plant defense, at the interface of canonical dimer-partners. Hemagglutination studies, enzyme kinetics, isothermal titration calorimetry and molecular dynamics showed that the lectin is specific to N-acetyl D-galactosamine, galactose and lactose in decreasing order, and alpha-amylase inhibitor. (C) 2014 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	Copy right for this article belongs to the ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Keywords:	Butea monosperma; Gamma aminobutyric acid; Glycosylation; Plant defense; alpha-amylase; Anti-fungal lectin
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	19 Jul 2015 09:41
Last Modified:	22 Feb 2019 05:45
URI:	http://eprints.iisc.ac.in/id/eprint/51892

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