Polypyridyl iron(II) complexes showing remarkable photocytotoxicity in visible light

Garai, Aditya and Basu, Uttara and Pant, Ila and Kondaiah, Paturu and Chakravarty, Akhil R (2015) Polypyridyl iron(II) complexes showing remarkable photocytotoxicity in visible light. In: JOURNAL OF CHEMICAL SCIENCES, 127 (4). pp. 609-618.

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Abstract

Iron(II) complexes of polypyridyl ligands (B), viz. Fe(B)(2)]Cl-2 (1 and 2) of N, N, N-donor 2-(2-pyridyl)-1,10-phenanthroline (pyphen in 1) and 3-(pyridin-2-yl)dipyrido3,2-a:2',3'-c]phenazine (pydppz in 2), are prepared and characterized. They are 1:2 electrolytes in aqueous DMF. The diamagnetic complexes exhibit metal to ligand charge transfer band near 570 nm in DMF. The complexes are avid binders to calf thymus DNA giving binding constant (K (b)) values of similar to 10(6) M-1 suggesting significant intercalative DNA binding of the complexes due to presence of planar phenanthroline bases. Complex 2 exhibits significant photocytotoxicity in immortalized human keratinocyte cells HaCaT and breast cancer cell line MCF-7 giving IC50 values of 0.08 and 13 mu M in visible light (400-700 nm). Complex 2 shows only minor dark toxicity in HaCaT cells but is non-toxic in dark in MCF-7 cancer cells. The light-induced cellular damage follows apoptotic pathway on generation of reactive oxygen species as evidenced from the dichlorofluorescein diacetate (DCFDA) assay.

Item Type:	Journal Article
Publication:	JOURNAL OF CHEMICAL SCIENCES
Publisher:	INDIAN ACAD SCIENCES
Additional Information:	Copy right for this article belongs to the INDIAN ACAD SCIENCES, C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA
Keywords:	Iron; polypyridyl bases; DNA binding; photocytotoxicity; apoptosis
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	19 Jul 2015 09:21
Last Modified:	19 Jul 2015 09:21
URI:	http://eprints.iisc.ac.in/id/eprint/51829

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