B-Spline potential function for maximum a-posteriori image reconstruction in fluorescence microscopy

Dilipkumar, Shilpa (2015) B-Spline potential function for maximum a-posteriori image reconstruction in fluorescence microscopy. In: AIP ADVANCES, 5 (3).

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Abstract

An iterative image reconstruction technique employing B-Spline potential function in a Bayesian framework is proposed for fluorescence microscopy images. B-splines are piecewise polynomials with smooth transition, compact support and are the shortest polynomial splines. Incorporation of the B-spline potential function in the maximum-a-posteriori reconstruction technique resulted in improved contrast, enhanced resolution and substantial background reduction. The proposed technique is validated on simulated data as well as on the images acquired from fluorescence microscopes (widefield, confocal laser scanning fluorescence and super-resolution 4Pi microscopy). A comparative study of the proposed technique with the state-of-art maximum likelihood (ML) and maximum-a-posteriori (MAP) with quadratic potential function shows its superiority over the others. B-Spline MAP technique can find applications in several imaging modalities of fluorescence microscopy like selective plane illumination microscopy, localization microscopy and STED. (C) 2015 Author(s).

Item Type:	Journal Article
Publication:	AIP ADVANCES
Publisher:	AMER INST PHYSICS
Additional Information:	Copy right for this article belongs to the AMER INST PHYSICS, 1305 WALT WHITMAN RD, STE 300, MELVILLE, NY 11747-4501 USA
Keywords:	OPTICAL-SECTIONING MICROSCOPY; INTERPOLATION METHODS; RESTORATION; DISTRIBUTIONS; ALGORITHMS; CELLS
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	06 May 2015 05:15
Last Modified:	06 May 2015 05:15
URI:	http://eprints.iisc.ac.in/id/eprint/51488

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