Lobe-specific Expression of Phosphodiesterase 5 in Rat Prostate

Wang, Lin and Zhang, Xiaoyu and Wang, Guifang and Visweswariah, Sandhya S and Lin, Guiting and Xin, Zhongcheng and Lue, Tom F. and Lin, Ching-Shwun (2015) Lobe-specific Expression of Phosphodiesterase 5 in Rat Prostate. In: UROLOGY, 85 (3).

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Abstract

OBJECTIVE To investigate the level and location of phosphodiesterase 5 (PDE5) expression in rat prostate. METHODS The ventral, dorsal, and lateral lobes of rat prostate were examined for PDE5 expression by Western blotting. Intact rat urogenital complex, including the urinary bladder and accessory reproductive glands, was examined for PDE5 expression by immunohistochemistry. Individual prostatic lobes were further examined by immunofluorescence for expression of PDE5, alpha-smooth muscle actin, and rat endothelial cell antigen. RESULTS Western blot analysis showed that PDE5 was expressed at a significantly lower level in dorsal lobe (DL) than in ventral lobe (VL) or lateral lobe (LL). Immunohistochemistry and immunofluorescence analyses showed that PDE5 was expressed in both acinar epithelium and periacinar smooth muscle. However, although similar levels of smooth muscle PDE5 expression were observed in all 3 prostatic lobes, significantly lower level of epithelial PDE5 expression was found in DL compared with VL or LL. In prostatic blood vessels, PDE5 expression was clearly visible in the endothelium but not as easily detectable in the smooth muscle. CONCLUSION PDE5 was expressed in the acinar epithelium and periacinar smooth muscle of rat prostate. However, the epithelial PDE5 expression was significantly less in DL than in VL or LL. Regardless, the acinar wall, not the blood vessel wall, is the predominant PDE5 expression site in rat prostate. (C) 2015 Elsevier Inc.

Item Type:	Journal Article
Publication:	UROLOGY
Publisher:	ELSEVIER SCIENCE INC
Additional Information:	Copy right for this article belongs to the ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Keywords:	LOWER URINARY-TRACT; CGMP-BINDING; FUNCTIONAL-ACTIVITY; SYMPTOMS SECONDARY; INHIBITORS; HYPERPLASIA; PDE5; LOCALIZATION; METAANALYSIS; ISOENZYMES
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	29 Apr 2015 04:51
Last Modified:	29 Apr 2015 04:51
URI:	http://eprints.iisc.ac.in/id/eprint/51446

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