Ozden, Sehmus and Narayanan, Tharangattu N and Tiwary, Chandra S and Dong, Pei and Hart, Amelia HC and Vajtai, Robert and Ajayan, Pulickel M (2015) 3D Macroporous Solids from Chemically Cross-linked Carbon Nanotubes. In: SMALL, 11 (6). pp. 688-693.
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Abstract
We demonstrate a new technique to generate multiple light-sheets for fluorescence microscopy. This is possible by illuminating the cylindrical lens using multiple copies of Gaussian beams. A diffraction grating placed just before the cylindrical lens splits the incident Gaussian beam into multiple beams traveling at different angles. Subsequently, this gives rise to diffraction-limited light-sheets after the Gaussian beams pass through the combined cylindrical lens-objective sub-system. Direct measurement of field at and around the focus of objective lens shows multi-sheet pattern with an average thickness of 7.5 μm and inter-sheet separation of 380 μm. Employing an independent orthogonal detection sub-system, we successfully imaged fluorescently-coated yeast cells (≈4 μm) encaged in agarose gel-matrix. Such a diffraction-limited sheet-pattern equipped with dedicated detection system may find immediate applications in the field of optical microscopy and fluorescence imaging. © 2015 Optical Society of America
Item Type: | Journal Article |
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Publication: | SMALL |
Publisher: | WILEY-V C H VERLAG GMBH |
Additional Information: | Copy right for this article belongs to the WILEY-V C H VERLAG GMBH, BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY |
Keywords: | RAMAN-SPECTROSCOPY; NITRIC-ACID; FUNCTIONALIZATION; OXIDATION; FILMS; TRANSPARENT; BUCKYPAPER; ELECTRODES; GRAPHENE; SPONGES |
Department/Centre: | Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy) |
Date Deposited: | 20 Apr 2015 07:22 |
Last Modified: | 20 Apr 2015 07:22 |
URI: | http://eprints.iisc.ac.in/id/eprint/51245 |
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