Jha, Prerana and Patric, Irene Rosita Pia and Shukla, Sudhanshu and Pathak, Pankaj and Pal, Jagriti and Sharma, Vikas and Thinagararanjan, Sivaarumugam and Santosh, Vani and Suri, Vaishali and Sharma, Mehar Chand and Arivazhagan, Arimappamagan and Suri, Ashish and Gupta, Deepak and Somasundaram, Kumaravel and Sarkar, Chitra (2014) Genome-wide methylation profiling identifies an essential role of reactive oxygen species in pediatric glioblastoma multiforme and validates a methylome specific for H3 histone family 3A with absence of G-CIMP/isocitrate dehydrogenase 1 mutation. In: NEURO-ONCOLOGY, 16 (12). pp. 1607-1617.
Full text not available from this repository. (Request a copy)Abstract
Background. Pediatric glioblastoma multiforme (GBM) is rare, and there is a single study, a seminal discovery showing association of histone H3.3 and isocitrate dehydrogenase (IDH) 1 mutation with a DNA methylation signature. The present study aims to validate these findings in an independent cohort of pediatric GBM, compare it with adult GBM, and evaluate the involvement of important functionally altered pathways. Methods. Genome-wide methylation profiling of 21 pediatric GBM cases was done and compared with adult GBM data (GSE22867). We performed gene mutation analysis of IDH1 and H3 histone family 3A (H3F3A), status evaluation of glioma cytosine-phosphate-guanine island methylator phenotype (G-CIMP), and Gene Ontology analysis. Experimental evaluation of reactive oxygen species (ROS) association was also done. Results. Distinct differences were noted between methylomes of pediatric and adult GBM. Pediatric GBM was characterized by 94 hypermethylated and 1206 hypomethylated cytosine-phosphate-guanine (CpG) islands, with 3 distinct clusters, having a trend to prognostic correlation. Interestingly, none of the pediatric GBM cases showed G-CIMP/IDH1 mutation. Gene Ontology analysis identified ROS association in pediatric GBM, which was experimentally validated. H3F3A mutants (36.4%; all K27M) harbored distinct methylomes and showed enrichment of processes related to neuronal development, differentiation, and cell-fate commitment. Conclusions. Our study confirms that pediatric GBM has a distinct methylome compared with that of adults. Presence of distinct clusters and an H3F3A mutation-specific methylome indicate existence of epigenetic subgroups within pediatric GBM. Absence of IDH1/G-CIMP status further indicates that findings in adult GBM cannot be simply extrapolated to pediatric GBM and that there is a strong need for identification of separate prognostic markers. A possible role of ROS in pediatric GBM pathogenesis is demonstrated for the first time and needs further evaluation.
Item Type: | Journal Article |
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Publication: | NEURO-ONCOLOGY |
Publisher: | OXFORD UNIV PRESS INC |
Additional Information: | Copy right for this article belongs to the OXFORD UNIV PRESS INC, JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA |
Keywords: | G-CIMP; H3F3A; IDH1; methylation; pediatric GBM; reactive oxygen species (ROS) |
Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
Date Deposited: | 04 Mar 2015 12:19 |
Last Modified: | 04 Mar 2015 12:19 |
URI: | http://eprints.iisc.ac.in/id/eprint/50965 |
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