Atomic resolution structure of the double mutant (K53,56M) of bovine pancreatic phospholipase $A_2$

Sekar, K and Yogavel, M and Gayathri, D and Velmurugan, D and Krishna, R and Poi, MJ and Dauter, Z and Dauter, M and Tsai, MD (2006) Atomic resolution structure of the double mutant (K53,56M) of bovine pancreatic phospholipase $A_2$. In: Acta Crystallographica, Section F: Structural Biology and Crystallization Communications, 62 (1). pp. 1-5.

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Abstract

The structure of the double mutant K53,56M has previously been refined at 1.9 A resolution using room-temperature data. The present paper reports the crystal structure of the same mutant K53,56M refined against 1.1 A data collected using synchrotron radiation. A total of 116 main-chain atoms from 29 residues and 44 side chains are modelled in alternate conformations. Most of the interfacial binding residues are found to be disordered and alternate conformations could be recognized. The second calcium ion-binding site residue Glu92 adopts two alternate conformations. The minor and major conformations of Glu92 correspond to the second calcium ion bound and unbound states.

Item Type:	Journal Article
Publication:	Acta Crystallographica, Section F: Structural Biology and Crystallization Communications
Publisher:	International Union of Crystallography
Additional Information:	The Copyright belongs to International Union of Crystallography.
Department/Centre:	Division of Interdisciplinary Sciences > Supercomputer Education & Research Centre Division of Information Sciences (Doesn't exist now) > BioInformatics Centre
Date Deposited:	18 Jan 2006
Last Modified:	19 Sep 2010 04:22
URI:	http://eprints.iisc.ac.in/id/eprint/5094

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