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The adenylyl cyclase Rv2212 modifies the proteome and infectivity of Mycobacterium bovis BCG

Pedroza-Roldan, Cesar and Aceves-Sanchez, Michel de Jesus and Zaveri, Anisha and Charles-Nino, Claudia and Elizondo-Quiroga, Darwin Eduardo and Hernandez-Gutierrez, Rodolfo and Allen, Kirk and Visweswariah, Sandhya S and Flores-Valdez, Mario Alberto (2015) The adenylyl cyclase Rv2212 modifies the proteome and infectivity of Mycobacterium bovis BCG. In: FOLIA MICROBIOLOGICA, 60 (1). pp. 21-31.

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Official URL: http://dx.doi.org/ 10.1007/s12223-014-0335-1


All organisms have the capacity to sense and respond to environmental changes. These signals often involve the use of second messengers such as cyclic adenosine monophosphate (cAMP). This second messenger is widely distributed among organisms and coordinates gene expression related with pathogenesis, virulence, and environmental adaptation. Genomic analysis in Mycobacterium tuberculosis has identified 16 adenylyl cyclases (AC) and one phosphodiesterase, which produce and degrade cAMP, respectively. To date, ten AC have been biochemically characterized and only one (Rv0386) has been found to be important during murine infection with M. tuberculosis. Here, we investigated the impact of hsp60-driven Rv2212 gene expression in Mycobacterium bovis Bacillus Calmette-Guerin (BCG) during growth in vitro, and during macrophage and mice infection. We found that hsp60-driven expression of Rv2212 resulted in an increased capacity of replication in murine macrophages but an attenuated phenotype in lungs and spleen when administered intravenously in mice. Furthermore, this strain displayed an altered proteome mainly affecting proteins associated with stress conditions (bfrB, groEL-2, DnaK) that could contribute to the attenuated phenotype observed in mice.

Item Type: Journal Article
Publisher: SPRINGER
Additional Information: Copyright for this article belongs to the SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 21 Jan 2015 04:36
Last Modified: 21 Jan 2015 04:36
URI: http://eprints.iisc.ac.in/id/eprint/50707

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