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Indigenous Development of an Imaging Flow Cytometer for Clinical and Biological Applications

Kalyan, Veerendra J and Srinivasan, Rajesh and Gorthi, Sai Siva (2014) Indigenous Development of an Imaging Flow Cytometer for Clinical and Biological Applications. In: Conferene on Light and Its Interactions with Matter, MAR 19-21, 2014, Natl Inst Technol, Kerala, INDIA, pp. 611-615.

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Official URL: http://dx.doi.org/10.1063/1.4898306


Flow cytometry is a benchmark technique used for basic research and clinical diagnosis of various diseases. Despite being a high-throughput technique, it fails in capturing the morphology of cells being analyzed. Imaging flow cytometry is a combination of flow-cytometry and digital microscopy, which offers advantages of both the techniques. In this paper, we report on the development of an indigenous Imaging Flow Cytometer, realized with the combination of Optics, Microfluidics, and High-speed imaging. A custom-made bright-field transmission microscope is used to capture images of cells flowing across the microfluidic device. High-throughput morphological analysis on suspension of yeast cells is presented.

Item Type: Conference Proceedings
Series.: AIP Conference Proceedings
Additional Information: Copyright for this article belongs to the AMER INST PHYSICS, 2 HUNTINGTON QUADRANGLE, STE 1NO1, MELVILLE, NY 11747-4501 USA
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 18 Jan 2015 06:49
Last Modified: 18 Jan 2015 06:49
URI: http://eprints.iisc.ac.in/id/eprint/50702

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