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Infection of human amniotic and endothelial cells by Japanese encephalitis virus: Increased expression of HLA-F

Kumar, Gaurav and Date, Onkar Sanjay and Kim, Kwang Sik and Manjunath, Ramanathapuram (2014) Infection of human amniotic and endothelial cells by Japanese encephalitis virus: Increased expression of HLA-F. In: VIROLOGY, 471 . pp. 29-37.

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Official URL: http://dx.doi.org/ 10.1016/j.virol.2014.09.022


Productive infection of human amniotic and endothelial cell lines with Japanese encephalitis virus (JEV) was established leading to the induction of NF kappa B and HLA-F, a non-classical MHC molecule. Induction of the HLA-F gene and protein in JEV-infected cells was shown to be NF kappa B dependent since it was blocked by inhibitors of NF kappa B activation. ShRNA targeting lentivirus-mediated stable knockdown of the p65 subunit of NF kappa B inhibited JEV-mediated induction of HLA-F both in the amniotic cell line, AV-3 as well as the human brain microendothelial cell line, HBMEC. The induction of HLA-F by treatment of AV-3 with TNF-alpha was also inhibited by ShRNA mediated knockdown of NF kappa B. TNF-alpha treatment of HEK293T cells that were transfected with reporter plasmids under the control of HLA-F enhancer A elements resulted in significant transactivation of the luciferase reporter gene. NF kappa B-mediated induction of HLA-F following JEV infection and TNF-alpha exposure is being suggested for the first time. (C) 2014 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Publication: VIROLOGY
Additional Information: Copyright for this article belongs to the ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
Keywords: JEV; TNF-alpha; Amniotic; Endothelial cells; NF kappa B mediated HLA-F induction
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 12 Jan 2015 05:00
Last Modified: 12 Jan 2015 05:00
URI: http://eprints.iisc.ac.in/id/eprint/50567

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