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Involvement of S-cerevisiae Rpb4 in subset of pathways related to transcription elongation

Deshpande, Swati M and Sadhale, Parag P and Vijayraghavan, Usha (2014) Involvement of S-cerevisiae Rpb4 in subset of pathways related to transcription elongation. In: GENE, 545 (1). pp. 126-131.

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Official URL: http://dx.doi.org/10.1016/j.gene.2014.04.061


Yeast Rpb4, a subunit of RNA pol II is not essential for viability but is involved in multiple cellular phenotypes such as temperature sensitivity, enhanced pseudohyphal morphology, and decreased sporulation. Both in vivo and in vitro studies strongly support involvement of Rpb4 in transcription initiation, while its role in transcription elongation is not entirely consistent. Here we show that Rpb4 is not required for recruitment of RNA pol II on the coding region of YLR454w, a representative long gene. Yet we find strong genetic interaction of rpb4 Delta with mutants in many transcription elongation factors such as Paf1, Spt4, Dst1, Elp3 and Rpb9. We demonstrate that, Rpb4 interacts functionally with Paf1 to affect the transcription elongation of the FKS1 gene. Our results suggest that while Rpb4 is not required for general transcription elongation, it could support transcription elongation for specific of class of genes by interaction with other elongation factors. (C) 2014 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Publication: GENE
Additional Information: Copyright for this article belongs to the ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Keywords: Rpb4; Transcription elongation; Elongation factors; Synthetic lethality; PKC pathway
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 22 Jul 2014 11:45
Last Modified: 22 Jul 2014 11:45
URI: http://eprints.iisc.ac.in/id/eprint/49503

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