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Rv1027c-Rv1028c encode functional KdpDE two - Component system in Mycobacterium tuberculosis

Agrawal, Ruchi and Saini, Deepak Kumar (2014) Rv1027c-Rv1028c encode functional KdpDE two - Component system in Mycobacterium tuberculosis. In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 446 (4). pp. 1172-1178.

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2014.03.066


In Mycobacterium tuberculosis Rv1027c-Rv1028c genes are predicted to encode KdpDE two component system, which is highly conserved across all bacterial species. Here, we show that the system is functionally active and KdpD sensor kinase undergoes autophosphorylation and transfers phosphoryl group to KdpE, response regulator protein. We identified His(642) and Asp(52) as conserved phosphorylation sites in KdpD and KdpE respectively and by SPR analysis confirmed the physical interaction between them. KdpD was purified with prebound divalent ions and their importance in phosphorylation was established using protein refolding and ion chelation approaches. Genetically a single transcript encoded both KdpD and KdpE proteins. Overall, we report that M. tuberculosis KdpDE system operates like a canonical two component system. (C) 2014 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Additional Information: copyright for this article belongs to ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
Keywords: Two component system; Mycobacterium tuberculosis; Sensor kinase; Response regulator; KdpDE
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 12 Jun 2014 10:00
Last Modified: 12 Jun 2014 10:00
URI: http://eprints.iisc.ac.in/id/eprint/49178

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