Evaluation of Brugia malayi sheath protein (Shp-1) as a diagnostic antigen for human lymphatic filariasis

Jawaharlal, Jeya Prita Parasurama and Ravishankaran, Rajendran and Shridharan, Radhika Nagamangalam and Lawrence, Ansel Vishal and Karande, Anjali Anoop and Perumal, Kaliraj (2014) Evaluation of Brugia malayi sheath protein (Shp-1) as a diagnostic antigen for human lymphatic filariasis. In: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 78 (3). pp. 249-254.

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Abstract

Lymphatic filariasis is the second leading cause of permanent long-term disability globally and control of this disease needs efficient diagnostic methods. In this study, abundantly expressing microfilarial sheath protein (Shp-1) from Brugia malayi was characterized as a filarial diagnostic candidate using samples from different clinical population. Monoclonal antibodies were developed against E. coil expressed recombinant Shp-1 in order to assess its efficiency in filarial antigen detection assay system. Endemic Normal (EN, n = 170), asymptomatic microfilaeremics (MF, n = 65), symptomatic chronic pathology (CP, n = 45) and non endemic normal (NEN, n = 10) sera were analyzed by antigen capture enzyme-linked immunosorbent assay. Of the 290 individuals, all MF individuals (both brugian and bancroftian) were positive in this assay followed by CP and EN. When compared with SXP-1 and Og4C3 antigen assays, all assays detected Wb MF correctly, Bm MF was detected by Shp-1 and SXP-1 assays, and only Shp-1 was able to detect EN (12%) and CP (29%). Results showed that this assay may be useful for monitoring prior to mass drug administration. (c) 2014 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Publication:	DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Publisher:	ELSEVIER SCIENCE INC
Additional Information:	Copyright for this article belongs to the ELSEVIER SCIENCE INC, USA
Keywords:	Lymphatic filariasis; Shp-1; Sheath protein; Monoclonal antibodies; Wuchereria bancrofti; Brugia malayi; ELISA
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	11 Apr 2014 09:57
Last Modified:	11 Apr 2014 09:57
URI:	http://eprints.iisc.ac.in/id/eprint/48834

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