The N-terminal region containing the zinc finger domain of tobacco streak virus coat protein is essential for the formation of virus-like particles

Mathur, Chhavi and Mohan, Kalyani and Rani, Usha TR and Reddy, Krishna M and Savithri, Handanahal S (2014) The N-terminal region containing the zinc finger domain of tobacco streak virus coat protein is essential for the formation of virus-like particles. In: ARCHIVES OF VIROLOGY, 159 (3). pp. 413-423.

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Abstract

Tobacco streak virus (TSV), a member of the genus Ilarvirus (family Bromoviridae), has a tripartite genome and forms quasi-isometric virions. All three viral capsids, encapsidating RNA 1, RNA 2 or RNA 3 and subgenomic RNA 4, are constituted of a single species of coat protein (CP). Formation of virus-like particles (VLPs) could be observed when the TSV CP gene was cloned and the recombinant CP (rCP) was expressed in E. coli. TSV VLPs were found to be stabilized by Zn2+ ions and could be disassembled in the presence of 500 mM CaCl2. Mutational analysis corroborated previous studies that showed that an N-terminal arginine-rich motif was crucial for RNA binding; however, the results presented here demonstrate that the presence of RNA is not a prerequisite for assembly of TSV VLPs. Instead, the N-terminal region containing the zinc finger domain preceding the arginine-rich motif is essential for assembly of these VLPs.

Item Type:	Journal Article
Publication:	ARCHIVES OF VIROLOGY
Publisher:	SPRINGER WIEN
Additional Information:	Copyright for this article belongs to the SPRINGER WIEN, SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	11 Apr 2014 09:53
Last Modified:	11 Apr 2014 09:54
URI:	http://eprints.iisc.ac.in/id/eprint/48831

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