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Optical microscopy methods for understanding learning and memory

Singh, Aditya and Kumar, Suraj and Singh, Vikram Pal and Balaji, J (2013) Optical microscopy methods for understanding learning and memory. In: CURRENT SCIENCE, 105 (11). pp. 1537-1548.

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Official URL: http://www.currentscience.ac.in/Volumes/105/11/153...


Structural dynamics of dendritic spines is one of the key correlative measures of synaptic plasticity for encoding short-term and long-term memory. Optical studies of structural changes in brain tissue using confocal microscopy face difficulties of scattering. This results in low signal-to-noise ratio and thus limiting the imaging depth to few tens of microns. Multiphoton microscopy (MpM) overcomes this limitation by using low-energy photons to cause localized excitation and achieve high resolution in all three dimensions. Multiple low-energy photons with longer wavelengths minimize scattering and allow access to deeper brain regions at several hundred microns. In this article, we provide a basic understanding of the physical phenomena that give MpM an edge over conventional microscopy. Further, we highlight a few of the key studies in the field of learning and memory which would not have been possible without the advent of MpM.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to the INDIAN ACAD SCIENCES, INDIA
Keywords: Learning; memory; optical microscopy; two-photon excitation
Department/Centre: Division of Biological Sciences > Centre for Neuroscience
Date Deposited: 17 Feb 2014 05:34
Last Modified: 17 Feb 2014 05:34
URI: http://eprints.iisc.ac.in/id/eprint/48380

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