RECQL4 and p53 potentiate the activity of polymerase and maintain the integrity of the human mitochondrial genome

Gupta, Shruti and De, Siddharth and Srivastava, Vivek and Hussain, Mansoor and Kumari, Jyoti and Muniyappa, K and Sengupta, Sagar (2014) RECQL4 and p53 potentiate the activity of polymerase and maintain the integrity of the human mitochondrial genome. In: CARCINOGENESIS, 35 (1). pp. 34-45.

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Abstract

Germline mutations in RECQL4 and p53 lead to cancer predisposition syndromes, Rothmund-Thomson syndrome (RTS) and Li-Fraumeni syndrome (LFS), respectively. RECQL4 is essential for the transport of p53 to the mitochondria under unstressed conditions. Here, we show that both RECQL4 and p53 interact with mitochondrial polymerase (Pol gamma A/B2) and regulate its binding to the mitochondrial DNA (mtDNA) control region (D-loop). Both RECQL4 and p53 bind to the exonuclease and polymerase domains of Pol gamma A. Kinetic constants for interactions between Pol gamma A-RECQL4, Pol gamma A-p53 and Pol gamma B-p53 indicate that RECQL4 and p53 are accessory factors for Pol gamma A-Pol gamma B and Pol gamma A-DNA interactions. RECQL4 enhances the binding of Pol gamma A to DNA, thereby potentiating the exonuclease and polymerization activities of Pol gamma A/B2. To investigate whether lack of RECQL4 and p53 results in increased mitochondrial genome instability, resequencing of the entire mitochondrial genome was undertaken from multiple RTS and LFS patient fibroblasts. We found multiple somatic mutations and polymorphisms in both RTS and LFS patient cells. A significant number of mutations and polymorphisms were common between RTS and LFS patients. These changes are associated with either aging and/or cancer, thereby indicating that the phenotypes associated with these syndromes may be due to deregulation of mitochondrial genome stability caused by the lack of RECQL4 and p53. Summary: The biochemical mechanisms by which RECQL4 and p53 affect mtDNA replication have been elucidated. Resequencing of RTS and LFS patients' mitochondrial genome reveals common mutations indicating similar mechanisms of regulation by RECQL4 and p53.

Item Type:	Journal Article
Publication:	CARCINOGENESIS
Publisher:	OXFORD UNIV PRESS
Additional Information:	copyright for this article belongs to OXFORD UNIV PRESS, ENGLAND
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	27 Jan 2014 06:30
Last Modified:	27 Jan 2014 06:30
URI:	http://eprints.iisc.ac.in/id/eprint/48249

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