Enhancing Surface Coverage and Growth in Layer-by-Layer Assembly of Protein Nanoparticles

Mohanta, Vaishakhi and Patil, Satish (2013) Enhancing Surface Coverage and Growth in Layer-by-Layer Assembly of Protein Nanoparticles. In: LANGMUIR, 29 (43). pp. 13123-13128.

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Abstract

Thin films of bovine serum albumin (BSA) nanoparticles are fabricated via layer-by-layer assembly. The surface of BSA nanoparticles have two oppositely acting functional groups on the surface: amine (NH2) and carboxylate (COO-). The protonation and deprotonation of these functional groups at different pH vary the charge density on the particle surface, and entirely different growth can be observed by varying the nature of the complementary polymer and the pH of the particles. The complementary polymers used in this study are poly(dimethyldiallylammonium chloride) (PDDAC) and poly(acrylic acid) (PAA). The assembly of BSA nanoparticles based on electrostatic interaction with PDDAC suffers from the poor loading of the nanoparticles. The assembly with PAA aided by a hydrogen bonding interaction shows tremendous improvement in the growth of the assembly over PDDAC. Moreover, the pH of the BSA nanoparticles was observed to affect the loading of nanoparticles in the LbL assembly with PAA significantly.

Item Type:	Journal Article
Publication:	LANGMUIR
Publisher:	AMER CHEMICAL SOC
Additional Information:	copyright for this article belongs to AMER CHEMICAL SOC USA
Department/Centre:	Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited:	12 Dec 2013 11:48
Last Modified:	12 Dec 2013 11:48
URI:	http://eprints.iisc.ac.in/id/eprint/47887

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