MRT Letter: Light Sheet Based Imaging Flow Cytometry on a Microfluidic Platform

Regmi, Raju and Mohan, Kavya and Mondal, Partha P (2013) MRT Letter: Light Sheet Based Imaging Flow Cytometry on a Microfluidic Platform. In: MICROSCOPY RESEARCH AND TECHNIQUE, 76 (11). pp. 1101-1107.

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Abstract

We propose a light sheet based imaging flow cytometry technique for simultaneous counting and imaging of cells on a microfluidic platform. Light sheet covers the entire microfluidic channel and thus omits the necessity of flow focusing and point scanning based technology. Another advantage lies in the orthogonal detection geometry that totally cuts-off the incident light, thereby substantially reducing the background in the detection. Compared to the existing state-of-art techniques the proposed technique shows marked improvement. Using fluorescently-coated Saccharomyces cerevisiae cells we have recorded cell counting with throughput as high as 2,090 cells/min in the low flow rate regime and were able to image the individual cells on-the-go. Overall, the proposed system is cost-effective and simple in channel geometry with the advantage of efficient counting in operational regime of low laminar flow. This technique may advance the emerging field of microfluidic based cytometry for applications in nanomedicine and point of care diagnostics. Microsc. Res. Tech. 76:1101-1107, 2013. (c) 2013 Wiley Periodicals, Inc.

Item Type:	Editorials/Short Communications
Publication:	MICROSCOPY RESEARCH AND TECHNIQUE
Publisher:	WILEY-BLACKWELL
Additional Information:	copyright for this article belongs to WILEY-BLACKWELL
Keywords:	fluorescence; imaging; cytometry; light-sheet microscopy
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	26 Nov 2013 15:16
Last Modified:	26 Nov 2013 15:16
URI:	http://eprints.iisc.ac.in/id/eprint/47786

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