Imaging Intracellular Zinc by Using a Glyoxal Bis(4-methyl-4-phenyl-3-thiosemicarbazone) Ligand

Palanimuthu, Duraippandi and Shinde, Sridevi Vijay and Dayal, Disha and Somasundaram, Kumaravel and Samuelson, Ashoka G (2013) Imaging Intracellular Zinc by Using a Glyoxal Bis(4-methyl-4-phenyl-3-thiosemicarbazone) Ligand. In: EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2013 (20). pp. 3542-3549.

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Abstract

The ligand glyoxal bis(4-methyl-4-phenyl-3-thiosemicarbazone) (GTSCH2) is shown to be a selective fluorescence turn-on sensor for zinc ions (Zn2+). This sensor is easy to synthesize, exhibits excellent sensitivity and selectivity towards Zn2+ over other physiologically relevant cations, and has sub-nanomolar binding affinity. It displays maximum fluorescence response to Zn2+ when the metal/ligand ratio is 1:1 and displays stable fluorescence over a broad pH range. The potential of GTSCH2 to image Zn2+ inside the cell was demonstrated in MCF-7 cells (human breast cancer cell line) by using flow cytometry and confocal fluorescence microscopy. Cell viability studies reveal that the probe is biocompatible and suitable for cellular applications.

Item Type:	Journal Article
Publication:	EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
Publisher:	WILEY-V C H VERLAG GMBH
Additional Information:	copyright for this article belongs to WILEY
Keywords:	Zinc; Thiosemicarbazones; Imaging agents; Fluorescence; Sensors
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	14 Nov 2013 10:48
Last Modified:	14 Nov 2013 10:48
URI:	http://eprints.iisc.ac.in/id/eprint/47758

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