Taylor series expansion based multidimensional image reconstruction for confocal and 4pi microscopy

Dilipkumar, Shilpa and Mondal, Partha Pratim (2013) Taylor series expansion based multidimensional image reconstruction for confocal and 4pi microscopy. In: Applied Physics Letters, 103 (7). 073702_1-073702_5.

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Abstract

We propose and experimentally demonstrate a three-dimensional (3D) image reconstruction methodology based on Taylor series approximation (TSA) in a Bayesian image reconstruction formulation. TSA incorporates the requirement of analyticity in the image domain, and acts as a finite impulse response filter. This technique is validated on images obtained from widefield, confocal laser scanning fluorescence microscopy and two-photon excited 4pi (2PE-4pi) fluorescence microscopy. Studies on simulated 3D objects, mitochondria-tagged yeast cells (labeled with Mitotracker Orange) and mitochondrial networks (tagged with Green fluorescent protein) show a signal-to-background improvement of 40% and resolution enhancement from 360 to 240 nm. This technique can easily be extended to other imaging modalities (single plane illumination microscopy (SPIM), individual molecule localization SPIM, stimulated emission depletion microscopy and its variants).

Item Type:	Journal Article
Publication:	Applied Physics Letters
Publisher:	American Institute of Physics
Additional Information:	Copyright of this article belongs to American Institute of Physics.
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	04 Oct 2013 05:37
Last Modified:	04 Oct 2013 05:37
URI:	http://eprints.iisc.ac.in/id/eprint/47490

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