Identification of immuno-dominant antigens of Trypanosoma evansi for detection of chronic trypanosomosis using experimentally infected equines

Yadav, SC and Kumar, R and Kumar, Vipin and Jaideep, * and Kumar, Ritesh and Gupta, AK and Bera, BC and Tatu, U (2013) Identification of immuno-dominant antigens of Trypanosoma evansi for detection of chronic trypanosomosis using experimentally infected equines. In: Research in Veterinary Science, 95 (2). pp. 522-528.

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Abstract

Trypanosoma evansi is the most extensively distributed trypanosome responsible for disease called surra in livestock in many countries including frequent outbreaks in India. The prevalence of this disease is most commonly reported by standard parasitological detection methods (SPDM); however, antibody ELISA is being in practice by locally produced whole cell lysate (WCL) antigens in many countries. In the present investigation, we attempted to identify and purify immuno dominant, infection specific trypanosome antigens from T. evansi proteome using experimentally infected equine serum by immuno blot. Three immuno dominant clusters of proteins i.e. 62-66 kDa, 52-55 kDa and 41-43 kDa were identified based on their consistent reactivity with donkey sequential serum experimentally infected T. evansi up to 280 days post infection (dpi). The protein cluster of 62-66 kDa was purified in bulk in native form and comparatively evaluated with whole cell lysate antigen (WCL). ELISA and immuno blot showed that polypeptide of this cluster is 100% sensitive in detection of early and chronic infection. Further, this protein cluster was also found immuno reactive against hyper immune serum raised against predominantly 66 kDa exo antigen, revealed that this is a common immunodominant moieties in proteome and secretome of T. evansi.

Item Type:	Journal Article
Publication:	Research in Veterinary Science
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Trypanosoma Evansi; Proteome; Immunodominant; Trypanosomosis; ELISA; Immunoblot
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	29 Oct 2013 05:42
Last Modified:	29 Oct 2013 05:42
URI:	http://eprints.iisc.ac.in/id/eprint/47472

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