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Charge Injection through Nanocomposite Electrode in Microfluidic Channel for Electrical Lysis of Biological Cells

Mishra, Madhusmita and Krishna, Anil and Chandra, Aman and Shenoy, BM and Hegde, GM and Mahapatra, Roy D (2013) Charge Injection through Nanocomposite Electrode in Microfluidic Channel for Electrical Lysis of Biological Cells. In: Conference on Frontiers in Biological Detection - From Nanosensors to Systems, FEB 02-03, 2013, San Francisco, CA.

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Official URL: http://dx.doi.org/ 10.1117/12.2012295


Several concepts have been developed in the recent years for nanomaterial based integrated MEMS platform in order to accelerate the process of biological sample preparation followed by selective screening and identification of target molecules. In this context, there exist several challenges which need to be addressed in the process of electrical lysis of biological cells. These are due to (i) low resource settings while achieving maximal lysis (ii) high throughput of target molecules to be detected (iii) automated extraction and purification of relevant molecules such as DNA and protein from extremely small volume of sample (iv) requirement of fast, accurate and yet scalable methods (v) multifunctionality toward process monitoring and (vi) downward compatibility with already existing diagnostic protocols. This paper reports on the optimization of electrical lysis process based on various different nanocomposite coated electrodes placed in a microfluidic channel. The nanocomposites are synthesized using different nanomaterials like Zinc nanorod dispersion in polymer. The efficiency of electrical lysis with various different electrode coatings has been experimentally verified in terms of DNA concentration, amplification and protein yield. The influence of the coating thickness on the injection current densities has been analyzed. We further correlate experimentally the current density vs. voltage relationship with the extent of bacterial cell lysis. A coupled multiphysics based simulation model is used to predict the cell trajectories and lysis efficiencies under various electrode boundary conditions as estimated from experimental results. Detailed in-situ fluorescence imaging and spectroscopy studies are performed to validate various hypotheses.

Item Type: Conference Proceedings
Series.: Proceedings of SPIE
Additional Information: Copyright of this article is belongs to SPIE-INT SOC OPTICAL ENGINEERING
Keywords: Nanocomposite; Electrical lysis; microfluidic; Multiphysics model; Debye layer; Cell trajectory
Department/Centre: Division of Mechanical Sciences > Aerospace Engineering(Formerly Aeronautical Engineering)
Date Deposited: 02 Oct 2013 06:10
Last Modified: 02 Oct 2013 06:10
URI: http://eprints.iisc.ac.in/id/eprint/47373

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