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Generation of extended light-sheets for single and multi-photon fluorescence microscopy

Purnapatra, Subhajit B and Mondal, Partha Pratim (2013) Generation of extended light-sheets for single and multi-photon fluorescence microscopy. In: APPLIED PHYSICS LETTERS, 103 (4).

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Official URL: http://dx.doi.org/10.1063/1.4816419


We theoretically propose and computationally demonstrate the generation of extended light-sheet for fluorescence microscopy. This is made possible by the introduction of a specially designed double-window spatial filter that allows the light to pass through the periphery and center of a cylindrical lens. When illuminated with a plane wave, the proposed filter results in an extended depth-of-focus along with side-lobes which are due to other interferences in the transverse focal plane. Computational studies show a maximum extension of light-sheet by 3.38 times for single photon excitation and 3.68 times for multiphoton excitation as compared to state-of-art single plane illumination microscopy system. This technique may facilitate the study of large biological specimens (such as Zebrafish embryo and tissue) with high spatial resolution and reduced photobleaching. (C) 2013 AIP Publishing LLC.

Item Type: Journal Article
Additional Information: Copyright of this article is belongs to AMER INST PHYSICS
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 24 Sep 2013 07:34
Last Modified: 24 Sep 2013 07:34
URI: http://eprints.iisc.ac.in/id/eprint/47300

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