Enhancing fluorescence signals from aluminium thin films and foils using polyelectrolyte multilayers

Prashanth, Gurusiddappa R and Goudar, Venkanagouda S and Raichur, Ashok M and Varma, Manoj M (2013) Enhancing fluorescence signals from aluminium thin films and foils using polyelectrolyte multilayers. In: Sensors and Actuators B: Chemical, 183 . pp. 496-503.

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Abstract

In this paper we investigate the application of polyelectrolyte multilayer (PEM) coated metal slides in enhancing fluorescence signal. We observed around eight-fold enhancement in fluorescence for protein incubated on PEM coated on aluminium mirror surface with respect to that of functionalized bare glass slides. The fluorescence intensities were also compared with commercially available FAST (R) slides (Whatman) offering 3D immobilization of proteins and the results were found to be comparable. We also showed that PEM coated on low-cost and commonly available aluminium foils also results in comparable fluorescence enhancement as sputtered aluminium mirrors. Immunoassay was also performed, using model proteins, on aluminium mirror as well as on aluminium foil based devices to confirm the activity of proteins. This work demonstrated the potential of PEMs in the large-scale, roll-to-roll manufacturing of fluorescence enhancements substrates for developing disposable, low-cost devices for fluorescence based diagnostic methods.

Item Type:	Journal Article
Publication:	Sensors and Actuators B: Chemical
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Polyelectrolyte Multilayers; Metal Enhanced Fluorescence; Diagnostic Devices; Low Cost biochip Manufacturing
Department/Centre:	Division of Electrical Sciences > Electronic Systems Engineering (Formerly Centre for Electronic Design & Technology) Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy) Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	19 Jul 2013 07:13
Last Modified:	19 Jul 2013 07:13
URI:	http://eprints.iisc.ac.in/id/eprint/46844

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