Employing denaturation for rapid electrochemical detection of myoglobin using TiO2 nanotubes

Mandal, Soumit S and Narayan, Karthik K and Bhattacharyya, Aninda J (2013) Employing denaturation for rapid electrochemical detection of myoglobin using TiO2 nanotubes. In: Journal of Materials Chemistry B, 1 (24). pp. 3051-3056.

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Abstract

An alternative antibody-free strategy for the rapid electrochemical detection of cardiac myoglobin has been demonstrated here using hydrothermally synthesized TiO2 nanotubes (Ti-NT). The denaturant induced unfolding of myoglobin led to easy access of the deeply buried electroactive heme center and thus the efficient reversible electron transfer from protein to electrode surface. The sensing performance of the Ti-NT modified electrodes were compared vis a vis commercially available titania and GCEs. The tubular morphology of the Ti-NT led to facile transfer of electrons to the electrode surface, which eventually provided a linear current response (obtained from cyclic voltammetry) over a wide range of Mb concentration. The sensitivity of the Ti-NT based sensor was remarkable and was equal to 18 mu A mg(-1) ml (detection limit = 50 nM). This coupled with the rapid analysis time of a few tens of minutes (compared to a few days for ELISA) demonstrates its potential usefulness for the early detection of acute myocardial infarction (AMI).

Item Type:	Journal Article
Publication:	Journal of Materials Chemistry B
Publisher:	Royal Society of Chemistry
Additional Information:	Copyright of this article belongs to Royal Society of Chemistry.
Department/Centre:	Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited:	11 Jul 2013 11:14
Last Modified:	11 Jul 2013 11:14
URI:	http://eprints.iisc.ac.in/id/eprint/46831

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