Growth kinetics and immune response of chimeric foot-and-mouth disease virus serotype `O' produced through replication competent mini genome of serotype Asia 1, 63/72, in BHK cell lines

Joshi, Rahul and Chandrasekar, S and Paul, Soumya and Chokkalingam, Ashok Kumar and Saravanan, T and Ahmad, Kakoli and Dechamma, HJ and Reddy, GR and Gopinath, SM and Das, DN and Shaila, MS and Bharath, GN and Suryanarayana, VVS (2013) Growth kinetics and immune response of chimeric foot-and-mouth disease virus serotype `O' produced through replication competent mini genome of serotype Asia 1, 63/72, in BHK cell lines. In: Virus Research, 173 (2). pp. 299-305.

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Abstract

Regular vaccinations with potent vaccine, in endemic countries and vaccination to live in non-endemic countries are the methods available to control foot-and-mouth disease. Selection of candidate vaccine strain is not only cumbersome but the candidate should grow well for high potency vaccine preparation. Alternative strategy is to generate an infectious cDNA of a cell culture-adapted virus and use the replicon for development of tailor-made vaccines. We produced a chimeric `O' virus in the backbone of Asia 1 and studied its characteristics. The chimeric virus showed high infectivity titre (>10(10)) in BHK 21 cell lines, revealed small plague morphology and there was no cross reactivity with antiserum against Asia I. The virus multiplies rapidly and reaches peak at 12 h post infection. The vaccine prepared with this virus elicited high antibody titres.

Item Type:	Journal Article
Publication:	Virus Research
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Foot-and-Mouth Disease; Vaccine; Chimeric Virus; Vaccine Strain; FMDV Minigenome
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	05 Jul 2013 06:05
Last Modified:	05 Jul 2013 06:05
URI:	http://eprints.iisc.ac.in/id/eprint/46798

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