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Mapping the apoptosis inducing domain of an immunomodulatory protein: glycodelin A

Ponnalagu, Devasena and Karande, Anjali A (2013) Mapping the apoptosis inducing domain of an immunomodulatory protein: glycodelin A. In: MOLECULAR AND CELLULAR BIOCHEMISTRY, 377 (1-2). pp. 131-141.

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Official URL: http://dx.doi.org/10.1007/s11010-013-1578-x


Glycodelin A (GdA) is a dimeric glycoprotein synthesized by the human endometrium under progesterone regulation. Based on the high sequence similarity with beta-lactoglobulin, it is placed under the lipocalin superfamily. The protein is one of the local immunomodulators present at the feto-maternal interface which affects both the innate as well as the acquired arms of the immune system, thereby bringing about successful establishment and progression of pregnancy. Our previous studies revealed that the domain responsible for the immunosuppressive activity of glycodelin lies on its protein backbone and the glycans modulate the same. This study attempts to further delineate the apoptosis inducing region of GdA. Our results demonstrate that the stretch of amino acid sequence between Met24 to Leu105 is necessary and sufficient to inhibit proliferation of T cells and induce apoptosis in them. Further, within this region the key residues involved in harboring the activity were shown to be present between Asp52 and Ser65.

Item Type: Journal Article
Publisher: SPRINGER
Additional Information: Copyright for this article belongs to the SPRINGER, NETHERLANDS.
Keywords: Glycodelin; Lipocalin; Apoptosis; Functional domain; Glycans; Protein backbone
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 30 Apr 2013 11:24
Last Modified: 30 Apr 2013 11:24
URI: http://eprints.iisc.ac.in/id/eprint/46451

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